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Ligation of the Fas antigen stimulates chemokine secretion in pancreatic cancer cell line PANC‐1 1
Author(s) -
Shimada Mitsue,
Andoh Akira,
Araki Yoshio,
Fujiyama Yoshihide,
Bamba Tadao
Publication year - 2001
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1046/j.1440-1746.2001.02583.x
Subject(s) - chemokine , stimulation , interleukin 8 , immune system , secretion , microbiology and biotechnology , apoptosis , biology , cytokine , medicine , endocrinology , immunology , biochemistry
Background and Aim: The role of chemokines in the process of immune cell infiltration into pancreatic cancer tissue has been reported. In this study, we investigated the induction of chemokines (interleukin (IL)‐8 and monocyte chemoattractant protein (MCP)‐1) by Fas antigen (Ag)‐stimulation in a human pancreatic cancer cell line, PANC‐1. Methods: The chemokine secretion was evaluated by using an ELISA and a northern blot, and the activation of nuclear factor‐κB (NF‐κB) was assessed by using an electrophoretic gel mobility shift assay (EMSA). Results: The Fas antigen (Ag) stimulation clearly induced an increase in IL‐8 and MCP‐1 secretion in PANC‐1 cells. This effect was also observed at the mRNA level. The induction of chemokine secretion by Fas Ag stimulation required de novo gene expression and protein synthesis. The pretreatment with interferon (IFN)‐γ markedly enhanced the effects of Fas Ag stimulation; IFN‐γ pretreatment and Fas Ag stimulation synergistically induced not only apoptosis but also IL‐8 and MCP‐1 secretion. Flow cytometric analysis demonstrated that IFN‐γ significantly enhanced Fas Ag expression. In addition, Fas Ag stimulation actually evoked NF‐κB activation in this cell line. Conclusion: Our results indicate that Fas Ag stimulation can induce chemokine secretion in PANC‐1 cells, suggesting the contribution of Fas stimulation to the accumulation of immune cells in pancreatic cancer tissue.