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Portal hypertensive response to bradykinin in inflamed or cirrhotic rat livers is mediated by B 2 ‐type receptors
Author(s) -
LoureiroSilva MaurÍcio R,
Molina HercÍlia M,
Borges Durval R
Publication year - 2001
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1046/j.1440-1746.2001.02397.x
Subject(s) - bradykinin , medicine , endocrinology , portal venous pressure , agonist , portal hypertension , perfusion , receptor , bradykinin receptor , receptor antagonist , antagonist , cirrhosis
Background: We have shown that the portal hypertensive response to bradykinin in normal rats is mediated by B 2 receptors. Methods: By using isolated and exsanguinated rat liver perfusion, we studied the portal hypertensive response to bradykinin or des‐Arg 9 ‐bradykinin (B 1 agonist) in inflamed or cirrhotic rat livers. Livers were perfused with bovine serum albumin Krebs–Henseleit buffer (pH 7.4; 37°C) at a constant flow rate, in the absence or presence of des‐Arg 9 [Leu 8 ]‐bradykinin or HOE 140 (B 1 and B 2 receptor antagonists, respectively). Bradykinin (140 nmol) or des‐Arg 9 ‐bradykinin was injected as a bolus via the afferent route to the liver. Results: Basal perfusion pressure in liver‐cirrhotic rats was higher than in normal rats. In normal, inflamed, or liver‐cirrhotic rats, the presence of the B 1 antagonist did not change the portal hypertensive response to bradykinin, while the B 2 antagonist abolished this response. A 140‐nmol dose of des‐Arg 9 ‐bradykinin did not change the perfusion pressure; 700 nmol of this B 1 agonist produced an insignificant perfusion pressure increase. The perfusion pressure increase induced by bradykinin in cirrhotic livers was lower than in normal livers. Conclusions: The portal hypertensive response to bradykinin in inflamed or cirrhotic rat livers is mediated by B 2 receptors, but not B 1 receptors, and there is a contracting hyporeactivity to bradykinin in cirrhotic rat livers.