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Prevention of proteolysis in cold‐stored rat liver by addition of amino acids to the preservation solution
Author(s) -
Charrueau Christine,
BlondéCynober Françoise,
CoudrayLucas Colette,
Poupon Raoul,
Chaumeil JeanClaude,
Hannoun Laurent,
Cynober Luc
Publication year - 2000
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1046/j.1440-1746.2000.02310.x
Subject(s) - proteolysis , medicine , amino acid , biochemistry , enzyme , biology
Background: One process identified as detrimental in liver preservation is proteolysis. Methods: We tested the effects of adding antiproteolytic amino acids ( L ‐alanine, L ‐glutamine, L ‐histidine, L ‐leucine, L ‐methionine, L ‐phenylalanine, L ‐proline, L ‐tryptophan) to the preservation medium, in a model of reperfusion of 24 h cold‐stored rat livers. Results: During the preservation period, antiproteolytic amino acids inhibited the proteolysis observed in stored livers as shown by branched‐chain amino acid fluxes, which switched from release to uptake. During reperfusion, cold storage of lives without the addition of antiproteolytic amino acids resulted in a decrease in the total amino acid and branched‐chain amino acid uptake and a lower perfusion flow rate. The addition of antiproteolytic amino acids during liver storage resulted in the maintenance of total amino acid and branched‐chain amino acid uptake and a significant improvement in the perfusion flow rate during reperfusion. Conclusions: The presence of antiproteolytic amino acids in the preservation medium might be of interest in improving hepatic graft viability in transplantation.