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MULTIPLE STRAIN HELICOBACTER PYLORI INFECTION IN PATIENTS WITH DYSPEPSIA
Author(s) -
Achwan Wenny Astuti,
Muttaqin Zainul,
Isnaningsih Sri,
Sumoharjo Suwignyo
Publication year - 2000
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1046/j.1440-1746.2000.015012h16.x
Subject(s) - helicobacter pylori , biopsy , medicine , rapd , antrum , gastroenterology , gastric biopsy , helicobacter , helicobacter pylori infection , endoscopy , strain (injury) , pathology , microbiology and biotechnology , stomach , gastritis , biology , population , genetic diversity , environmental health
Objective Some author reported that infection by more than one strain of Helicobacter pylori in the same patient is not rare. The objective of this study is to explore the possibility of multiplicity of Helicobacter pylori strain in patients with dyspepsia using Random Amplified Polymorphic DNA (RAPD) technique. Material and Method Upper GI Endoscopy and gastric mucosal biopsy were done on 81 patiens with dyspepsia in Endoscopy Unit Department of Internal Medicine Mataram General Hospital. Gastric Mucosal biopsy was done on three different sites of antrum. Primary culture of Helicobacter pylori was done from each biopsy specimen separately. RAPD was done by Polymerase Chain Reaction using single primer (5’AACGCGCAAC3’) on the colonies grown from secondary culture of each biopsy specimen. Results Helicobacter pylori can be grown in 23 patients (28.3%). Helicobacter pylori was grown from single biopsy specimen in 17 patients, while in 6 patients from 2 or 3 biopsy specimens. Heterogenity of RAPD pattern was found in 3 cases, while in the other 3 cases RAPD patterns were identical. Pattern of antibiotic sensitivity of strains found in the same patients was identical. Conclusion: Infection with more than one strain with different genetic pattern can be found in patients with dyspepsia caused by Helicobacter pylori infection.