Premium
Increased mucosal production of granulocyte colony‐stimulating factor is related to a delay in neutrophil apoptosis in Inflammatory Bowel disease
Author(s) -
Ina Kenji,
Kusugami Kazuo,
Hosokawa Takehiko,
Imada Akira,
Shimizu Tatsuji,
Yamaguchi Takeo,
Ohsuga Masahiro,
Kyokane Kazuhiro,
Sakai Tetsu,
Nishio Yuji,
Yokoyama Yasuhisa,
Ando Takafumi
Publication year - 1999
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1046/j.1440-1746.1999.01807.x
Subject(s) - apoptosis , granulocyte , granulocyte macrophage colony stimulating factor , medicine , immunology , cytokine , granulocyte colony stimulating factor , acridine orange , inflammatory bowel disease , colony stimulating factor , incubation , biology , haematopoiesis , chemotherapy , biochemistry , disease , microbiology and biotechnology , stem cell
Tissue accumulation of polymorphonuclear neutrophils (PMN) in Inflammatory Bowel disease (IBD) might be, in part, due to a delay in apoptotic processes associated with the effects of their specific growth factors and inflammatory cytokines. We addressed this hypothesis by examining the activity of granulocyte colony‐stimulating factor (G‐CSF) and granulocyte–macrophage CSF (GM‐CSF) in the organ culture supernatants of colonic mucosal specimens and their regulatory effects on PMN apoptosis in patients with IBD. The contents of G‐CSF and GM‐CSF in the supernatants were measured by the enzyme‐linked immunosorbent assays and PMN apoptosis was evaluated by acridine orange/ethidium bromide staining, respectively. Mucosal specimens obtained from patients with active IBD exhibited higher levels of G‐CSF and GM‐CSF activity than controls. Notably, the levels of G‐CSF activity were approximately 1000‐fold higher than those of GM‐CSF activity. Freshly isolated PMN showed a time‐related increase in the proportion of cells with characteristic features of apoptosis when they were incubated with the culture medium alone and exposure of PMN to recombinant G‐CSF and GM‐CSF caused a concentration‐dependent inhibition of apoptosis. Incubation of PMN with the supernatants from patients with active IBD induced an inhibitory effect on PMN apoptosis; this effect was abrogated to a significant degree by pre‐incubation of the supernatants with anti‐G‐CSF serum. This study suggests that PMN apoptosis may be delayed under the influence of soluble mediators, especially G‐CSF, in the microenvironment of IBD‐affected mucosa, thus providing possible mechanisms for tissue accumulation of PMN in IBD.