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The novel histamine H 2 receptor antagonist FRG‐8813 prevents delay of wound repair induced by hydrogen peroxide in a rabbit gastric epithelial cell system
Author(s) -
SATO NOBUHIRO,
WATANABE SUMIO,
WANG XIANEN,
OSADA TARO,
TANAKA HIROSHI,
ITATSU TOMOKO,
MIYATA RYUKO,
WATANABE KENICHI,
SATO KENJI,
NAKAJIMA MIKAKO,
YAMASHINA SYUNPEI,
MATSUZAKI KENICHIRO,
MIWA HIROTO
Publication year - 1998
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1046/j.1440-1746.1998.01735.x
Subject(s) - wound healing , histamine , apoptosis , hydrogen peroxide , histamine h2 receptor , medicine , cell growth , antagonist , receptor antagonist , cell migration , cell , pharmacology , receptor , microbiology and biotechnology , cancer research , immunology , biochemistry , chemistry , biology
The effects of a novel histamine H 2 receptor antagonist (FRG‐8813) on the restoration process of gastric epithelial wounds were assessed using an in vitro wound healing model. FRG‐8813 (1, 10 mol/L) was added to a complete confluent monolayer cell sheet after artificial wounding. The restoration process was analysed by a time‐lapse video system and cell migration, proliferation and apoptosis were assessed. Hydrogen peroxide (1, 3 mmol/L) inhibited restoration after wounding by suppressing cell migration and proliferation and induced epithelial cell apoptosis around the wound. The addition of FRG‐8813 abolished the hydrogen peroxide‐induced retardation and prevented apoptosis, although FRG‐8813 itself did not enhance wound healing. FRG‐8813 may act as a radical scavenger as well as having an anti‐secretory action and may have favourable effects on peptic ulcer healing.