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1α,25‐Dihydroxyvitamin D 3 inhibits pro‐inflammatory cytokine and chemokine expression in human corneal epithelial cells colonized with Pseudomonas aeruginosa
Author(s) -
Xue MeiLang,
Zhu Hua,
Thakur Archana,
Willcox Mark
Publication year - 2002
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1046/j.1440-1711.80.4august.1.x
Subject(s) - chemokine , immune system , microbiology and biotechnology , pseudomonas aeruginosa , biology , interleukin 8 , cytokine , messenger rna , gene expression , cell culture , in vitro , immunology , gene , bacteria , biochemistry , genetics
The cytokines IL‐1β, IL‐6 and the chemokine IL‐8 are key mediators of host inflammation. 1α,25‐Dihydroxyvitamin D 3 (VD 3 ) has been shown to regulate host immune responses in vivo and in vitro . The purpose of this study was to investigate whether the addition of VD 3 to human corneal epithelial cells colonized with Pseudomonas aeruginosa altered the expression of IL‐1β, IL‐6 and IL‐8. An immortalized human corneal epithelial (HCE) cell line was used in this study. After growth to confluency, HCE cells were challenged with P. aeruginosa strain 6294 in the presence or absence of 10 −6 mol/L VD 3 for 4 h, 8 h and 12 h. Gene expression of IL‐1β, IL‐6 and IL‐8 was detected by reverse transcription‐PCR (RT‐PCR) from total RNA extracted from HCE cells. Protein concentrations of IL‐1β, IL‐6 and IL‐8 in culture supernatants were measured by ELISA. Addition of VD 3 to HCE cells colonized with P. aeruginosa significantly inhibited the expression of IL‐1β and IL‐8 mRNA and protein ( P < 0.05). Although the expression of IL‐6 mRNA was stimulated at 12 h post‐challenge ( P < 0.05), the expression of IL‐6 protein was inhibited at all time points after the addition of VD 3 . In conclusion, this study demonstrated that VD 3 inhibited the P. aeruginosa ‐induced expression of IL‐1β, IL‐6 and IL‐8 in HCE cells, suggesting that this vitamin may have the potential to become a novel anti‐inflammatory agent in ocular disease.