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Costimulation of T cell receptor‐triggered IL‐2 production by Jurkat T cells via fibroblast growth factor receptor 1 upon its engagement by CD56
Author(s) -
Kos Ferdynand J,
Chin Cynthia S
Publication year - 2002
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1046/j.1440-1711.2002.01098.x
Subject(s) - jurkat cells , neural cell adhesion molecule , microbiology and biotechnology , fibroblast growth factor receptor 1 , cytotoxic t cell , interleukin 21 , t cell , fibroblast growth factor receptor , biology , cd49b , cell adhesion molecule , il 2 receptor , receptor , chemistry , cell adhesion , fibroblast growth factor , cell , immunology , immune system , in vitro , biochemistry
Recent studies have demonstrated that neural cell adhesion molecule (NCAM) is involved in multiple adhesive interactions with several different classes of ligands on the cell surface and in the extracellular matrix. One of these ligands is fibroblast growth factor receptor (FGFR) that is expressed on neural cells. While it is known that CD56 is a molecular isoform of NCAM expressed on human NK cells and a subset of T cells, it remains poorly characterized, with its ligand unidentified. Therefore, we were prompted to examine if CD56 molecules on NK cells interact with FGFR expressed on T cells. We demonstrate that ligation of FGFR1β on J.C2‐14 Jurkat T cells by CD56 on fixed NK‐92 cells costimulates TCR/CD3‐triggered IL‐2 production. CD56‐binding mAbs inhibited the costimulatory effect of NK‐92 cells in 50−75%. Flow cytometric analysis and cell adhesion assays showed that FGFR1β/Fc and FGFR2β/Fc chimeric proteins bind to NK‐92 cells. The binding of FGFR1β/Fc protein to CD56 molecules was verified by immunoprecipitation of CD56 with anti‐CD56 mAb followed by Western blotting with FGFR1β/Fc. These findings suggest that ligation of FGFR1 by CD56 may contribute to the interaction between NK cells and T cells that we have postulated in our previous studies.