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Trypanosoma cruzi heat‐shock protein‐70 kDa, alone or fused to the parasite KMP11 antigen, induces functional maturation of murine dendritic cells
Author(s) -
Planelles L,
Thomas MC,
Pulgar M,
Marañón C,
Grabbe S,
López MC
Publication year - 2002
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1046/j.1440-1711.2002.01081.x
Subject(s) - trypanosoma cruzi , antigen , dendritic cell , microbiology and biotechnology , hsp70 , biology , heat shock protein , bone marrow , interleukin 12 , chemistry , immunology , parasite hosting , cytotoxic t cell , in vitro , biochemistry , gene , world wide web , computer science
We analyse the effect of Trypanosoma cruzi heat‐shock protein‐70 (HSP70) on the maturation of murine dendritic cells (DC) generated from bone marrow precursor cells. The results obtained show that HSP70, both alone and fused to the KMP11 antigen, as well as a HSP70 fragment, is capable of maturing murine DC. Mature DC have enhanced expression of IL12, TNF‐α cytokines, costimulation molecules and activation markers, showing a clear increase in the allostimulatory capacity. These findings suggest that T. cruzi HSP70 may be a very useful vehicle for developing DC‐based immunoprophylaxis and therapy against infections.