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Characterization of two TCR transgenic mouse lines specific for herpes simplex virus
Author(s) -
Mueller Scott N,
Heath William R,
McLain Julie D,
Carbone Francis R,
Jones Claerwen M
Publication year - 2002
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1046/j.1440-1711.2002.01071.x
Subject(s) - t cell receptor , herpes simplex virus , biology , cd8 , clone (java method) , virology , t cell , transgene , microbiology and biotechnology , genetically modified mouse , major histocompatibility complex , cytotoxic t cell , immune system , virus , immunology , in vitro , genetics , gene
To better understand the T cell‐mediated processes involved in the immune response to herpes simplex virus type 1 (HSV‐1) infection, two HSV‐specific T cell receptor (TCR) transgenic mouse lines were produced. These mice (gBT‐I.1 and gBT‐I.3) are MHC class I‐restricted and specific for the immunodominant peptide from HSV glycoprotein B (gB), gB 498–505 . Although derived from the same clone, the mice differ in the chromosomal location of the TCR transgenes and show marked differences in TCR α/β expression on both CD4 + and CD8 + cells in the thymus. Despite this, peripheral CD8 + T cells from both mice express equally high levels of the transgenic TCR and bind the K b gB 498–505 tetramer to the same degree. In concordance with this, both were shown to respond equally well in vitro upon stimulation with the gB 498–505 peptide or HSV‐infected cells. These data show that selection of broadly equivalent peripheral T‐cell subsets can occur in the presence of distinctly different thymic T‐cell subsets.