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Studying heterotrimeric G‐protein‐linked signal transduction using replication‐deficient adenoviruses
Author(s) -
Cummins Michelle M,
Poronnik Philip,
O’Mullane Lauren M,
Cook David I
Publication year - 2000
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1046/j.1440-1711.2000.00932.x
Subject(s) - heterotrimeric g protein , g protein , signal transduction , biology , g protein coupled receptor , microbiology and biotechnology , gtpase activating protein , transduction (biophysics) , gps2 , effector , receptor , membrane protein , vesicle associated membrane protein 8 , genetics , biochemistry , membrane
Plasma membrane‐spanning G‐protein‐linked receptors transduce approximately 60% of all extracellular stimuli in higher animals. Many G‐protein‐linked receptor pathways are yet to be elucidated, with the receptor, G‐protein or effector system as yet unidentified. In addition, many fundamental issues pertaining to G‐protein signalling remain unresolved, such as the factors governing the specificity of G‐protein receptor coupling and the control of signal amplitude in response to G‐protein activation. In order to address some of these issues, the use of replication‐deficient adenoviruses as gene transfer vectors for investigations of G‐protein signalling has been developed, facilitating dissection of G‐protein‐linked signal transduction pathways in an extensive range of cultured cells, as well as in vivo . The present review focuses on the versatility and utility of adenoviruses for the investigation of signalling by heterotrimeric G‐proteins and explores some of the recent advances in adenoviral technology as they relate to the study of signal transduction.