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Seeing is believing: Recent trends in the measurement of Ca 2+ in subcellular domains and intracellular organelles
Author(s) -
Monteith Gregory R
Publication year - 2000
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1046/j.1440-1711.2000.00920.x
Subject(s) - organelle , intracellular , cytosol , cytoplasm , microbiology and biotechnology , subcellular localization , mitochondrion , biology , chemistry , biophysics , biochemistry , enzyme
The role of Ca 2+ in the regulation of the cell cycle has been investigated mostly in studies assessing global cytosolic free Ca 2+ . Recent studies, however, have used unique techniques to assess Ca 2+ in subcellular organelles, such as mitochondria, and in discrete regions of the cytoplasm. These studies have used advanced fluorescence digital imaging techniques and Ca 2+ ‐sensitive fluorescence probes, and/or targeting of Ca 2+ ‐sensitive proteins to intracellular organelles. The present review describes the results of some of these studies and the techniques used. The novel techniques used to measure Ca 2+ in microdomains and intracellular organelles are likely to be of great use in future investigations assessing Ca 2+ homeostasis during the cell cycle.