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Expression of the interferon‐inducible chemokines MuMig and Crg‐2 following vaccinia virus infection in vivo
Author(s) -
Mahalingam Surendran,
Karupiah Gunasegaran
Publication year - 2000
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1046/j.1440-1711.2000.00894.x
Subject(s) - biology , vaccinia , interferon , northern blot , chemokine , virus , virology , microbiology and biotechnology , gene expression , gene , messenger rna , subfamily , in vivo , immunology , recombinant dna , immune system , genetics
MuMig ( m onokine i nduced by g amma interferon) and Crg‐2 ( c ytokine r esponsive g ene) are chemokines of the CXC subfamily. They share activity as T and NK cell chemoattractants. Crg‐2 has been shown to be inducible by IFN, TNF, IL‐1 and LPS, whereas the expression of MuMig is thought to be strictly dependent on IFN‐γ. In the present study, the kinetics of expression of the genes for MuMig and Crg‐2 were analysed by northern blot analysis in organs of normal mice and in groups of gene knockout mice deficient in IFN‐γ (IFN‐γ –/– ) or receptors for IFN‐α/β and IFN‐γ (double R –/– ; DR –/– ) after vaccinia virus infection. MuMig mRNA was not expressed in IFN‐γ –/– mice in all organs examined, whereas Crg‐2 mRNA levels were marginally reduced. In contrast, MuMig and Crg‐2 mRNA transcripts were completely abolished in the DR –/– mice.