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The use of carboxyfluorescein diacetate succinimidyl ester to determine the site, duration and cell type responsible for antigen presentation in vivo
Author(s) -
Mintern Justine,
Li Ming,
Davey Gayle M,
Blanas Effrossini,
Kurts Christian,
Carbone Francis R,
Heath William R
Publication year - 1999
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1046/j.1440-1711.1999.00868.x
Subject(s) - antigen , in vivo , cell , antigen presentation , presentation (obstetrics) , chemistry , microbiology and biotechnology , biology , immunology , t cell , biochemistry , medicine , immune system , genetics , radiology
This report examines the use of 5‐(and‐6)‐carboxyfluorescein diacetate succinimidyl ester (CFSE) to determine the site, duration and cell type responsible for antigen presentation in vivo . Evidence that CFSE‐labelled T cells can be used to determine where various types of antigens are presented, including auto‐antigens, oral antigens and cell‐associated foreign antigens, is provided. Using this technique, the length of time antigen is presented after acquisition by APC was measured. Finally, CFSE labelling was used to identify the origin of the APC responsible for different forms of antigen presentation.