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Stimulation of TNF‐α, IL‐1β and nitrite release from mouse cultured spleen cells and lavaged peritoneal cells by mastoparan M
Author(s) -
Wu TzongMing,
Chou TzChong,
Ding YuAn,
Li MingLiang
Publication year - 1999
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1046/j.1440-1711.1999.00847.x
Subject(s) - mastoparan , tumor necrosis factor alpha , nitrite , in vivo , microbiology and biotechnology , nitric oxide , endocrinology , chemistry , biology , immunology , medicine , biochemistry , receptor , g protein , organic chemistry , nitrate
Chemically synthesized mastoparan M, a tetradecapeptide toxin of venom (INLKAIAALAKKLL), was used in the experiments described. After addition of mastoparan M to cultures of mouse macrophages in vitro , tumour necrosis factor‐α (TNF‐α) and interleukin 1β (IL‐1β) were detected in the culture fluids by 12 h and their highest accumulation was observed by 24 h. Mastoparan M induced increases in both TNF‐α secretion and mRNA level at the same time. Nitrite levels, which reflect nitric oxide synthesis, were also found to increase in the macrophage cultures at 24 h after mastoparan M addition. In vivo studies showed that mastoparan M induced the formation and accumulation of TNF‐α, IL‐1β and nitrite in the peritoneal exudates of mice much faster at 90 min, 120 min and 180 min after mastoparan M injection, respectively. Similarly, significant increases in myeloperoxidase activity, a marker for neutrophil and macrophage content, were observed in the peritoneal lavage cells after intraperitoneal injection of mastoparan M. However, induction of nitrite by mastoparan M was completely inhibited by simultaneous addition of antimouse TNF‐α antibody to the macrophage cultures. These results suggest that modulation of both neutrophil and macrophage influx by mastoparan M may be conveyed through TNF‐α and IL‐1β secretion accompanied by nitrite formation.