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Caenorhabditis elegans DAF‐21 (HSP90) is characteristically and predominantly expressed in germline cells: Spatial and temporal analysis
Author(s) -
Inoue Tadao,
Takamura Katsumi,
Yamae Hisanori,
Ise Naoto,
Kawakami Manabu,
Tabuse Yo,
Miwa Johji,
Yamaguchi Yasunori
Publication year - 2003
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1046/j.1440-169x.2003.00706.x
Subject(s) - biology , caenorhabditis elegans , somatic cell , germline , complementary dna , monoclonal antibody , microbiology and biotechnology , heat shock protein , messenger rna , caenorhabditis , in situ hybridization , antigen , antibody , gene , genetics
Three monoclonal antibodies against antigens that exist in the Caenorhabditis elegans germ line have previously been described. In the present study, a full‐length mRNA for one of these antigens was isolated, and by sequencing its corresponding cDNA, it was predicted that the protein would show a high homology with the 90 kDa heat shock protein (HSP90) in other species, and with the protein of daf‐21 , a previously identified hsp90 homologue. The spatial and temporal distribution of the antigen (DAF‐21) was analyzed in C. elegans , and the localization of daf‐21 mRNA, as detected by in situ hybridization, agreed with that detected by the monoclonal antibody. Under normal conditions, daf‐21 mRNA is characteristically distributed in postembryonic germ cells derived from Z2 and Z3 cells in both hermaphrodites and males. Under heat stress conditions, however, daf‐21 mRNA was not only detected in germ cells, but also apparently expressed all over the body. In addition, the DAF‐21 protein seemed to be localized in the perinuclear region of somatic cells.