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Developmental expression patterns of α1H T‐type Ca 2+ channels during spermatogenesis and organogenesis in mice
Author(s) -
Son WeonYoung,
Han ChingTack,
Lee JaeHo,
Jung KyuYong,
Lee HyoungMin,
Choo YoungKug
Publication year - 2002
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1046/j.1440-169x.2002.00633.x
Subject(s) - biology , organogenesis , spermatogenesis , in situ hybridization , microbiology and biotechnology , messenger rna , sertoli cell , digoxigenin , embryo , endocrinology , gene , genetics
The objectives of the present study were to investigate the expression patterns of T‐type Ca 2+ channel mRNA during spermatogenesis and organogenesis in mice. Reverse transcription–polymerase chain reaction (RT–PCR) was performed to identify the subtypes of calcium channels present in the round spermatids isolated from mouse testes by flow cytometry. Transcripts of L‐type (α1D), non‐L‐type (α1E) and T‐type Ca 2+ channels were detected in round spermatids. Analysis of PCR products of T‐type Ca 2+ channels indicated that only α1H subunits were detected in round spermatids. The appearance and differential distribution of α1H T‐type Ca 2+ channel mRNA during mouse spermatogenesis and postimplantation embryogenesis (embryonic (E) days E9, E12, E15) were investigated by in situ hybridization with digoxigenin‐labeled RNA probes coupled with alkaline phosphatase detection. In testes from adult and immature mice (postnatal 2 and 3 weeks), α1H T‐type Ca 2+ channel mRNA was expressed in all developing germ cells and sertoli cells. On E9 and E12, tissues of the central nervous system, such as the telencephalon, expressed α1H T‐type Ca 2+ channel mRNA. On E15, signals were detected throughout all organs of the embryo. These findings indicate that the expression of α1H T‐type Ca 2+ channels is spatio‐temporally regulated during spermatogenesis and organogenesis.