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Possible role for the c‐ ski gene in the proliferation of myogenic cells in regenerating skeletal muscles of rats
Author(s) -
Soeta Chie,
Suzuki Michio,
Suzuki Shunichi,
Naito Kunihiko,
Tachi Chikashi,
Tojo Hideaki
Publication year - 2001
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1046/j.1440-169x.2001.00565.x
Subject(s) - myogenesis , skeletal muscle , desmin , regeneration (biology) , microbiology and biotechnology , biology , myocyte , cell growth , cardiotoxin , proliferating cell nuclear antigen , myod , gene expression , immunology , endocrinology , gene , immunohistochemistry , genetics , vimentin
Skeletal muscle regeneration after injury involves various processes, such as infiltration by inflammatory cells, the proliferation of satellite cells and fusion to myotubes. The c‐ ski nuclear protein has been implicated in the control of cell proliferation and/or terminal differentiation in the growth of skeletal muscle. However, there have been no reports concerning the involution of c‐ ski in the regeneration of injured skeletal muscle in mammals. A possible role for c‐ ski in the proliferation of myogenic cells in rat skeletal muscle during regeneration has been investigated with the assistance of in vitro experiments with L6 skeletal muscle cells. The expression levels of c‐ ski mRNA in regenerating tissues increased to approximately threefold that of intact tissues at 2 days after injury and decreased to normal levels at 2 weeks after injury. Many mononuclear cells among the Ski‐positive cells expressed desmin and proliferating cell nuclear antigen, indicating that Ski‐producing cells include the proliferating myogenic cells. The proliferation of L6 cells was significantly retarded by expression of the antisense ski gene. The results of the present study reveal that the c‐ ski gene plays an important role in the proliferation of myogenic cells in the regeneration of injured skeletal muscle.