Premium
Involvement of Frizzled‐10 in Wnt‐7a signaling during chick limb development
Author(s) -
Kawakami Yasuhiko,
Wada Naoyuki,
Nishimatsu Shinichiro,
Nohno Tsutomu
Publication year - 2000
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1046/j.1440-169x.2000.00545.x
Subject(s) - wnt signaling pathway , limb bud , frizzled , zone of polarizing activity , limb development , ectoderm , biology , apical ectodermal ridge , microbiology and biotechnology , sonic hedgehog , xenopus , morphogenesis , lrp6 , anatomy , embryogenesis , signal transduction , embryo , genetics , gene
The dorsal ectoderm of the limb bud is known to regulate anterior–posterior patterning as well as dorsal– ventral patterning during vertebrate limb morphogenesis. Wnt‐7a , expressed in the dorsal ectoderm, encodes a key molecule implicated in these events. In the present study, chicken frizzled‐10 ( Fz‐10 ) encoding a Wnt receptor was used to study mechanisms of Wnt‐7a signaling during chick limb patterning, because its expression is restricted to the posterior‐distal region of the dorsal limb bud. Fz‐10 transcripts colocalize with Sonic hedgehog ( Shh ) in the dorsal side of stages 18–23 chick limb buds. It was demonstrated that Fz‐10 interacts with Wnt‐7a to induce synergistically the expression of Wnt‐responsive genes, such as Siamois and Xnr3 , in Xenopus animal cap assays. In the chick limb bud, Fz‐10 expression is regulated by Shh and a signal from the dorsal ectoderm, presumably Wnt‐7a, but not by signals from the apical ectodermal ridge. These results suggest that Fz‐10 acts as a receptor for Wnt‐7a and has a positive effect on Shh expression in the chick limb bud.