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Application of efficient and specific gene transfer systems and organ culture techniques for the elucidation of mechanisms of epithelial– mesenchymal interaction in the developing gut
Author(s) -
Fukuda Kimiko,
Sakamoto Nobuyuki,
Narita Tomohiro,
Saitoh Kanako,
Kameda Takashi,
Iba Hideo,
Yasugi Sadao
Publication year - 2000
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1046/j.1440-169x.2000.00503.x
Subject(s) - mesenchymal stem cell , biology , electroporation , gene , embryonic stem cell , organ culture , microbiology and biotechnology , vertebrate , proventriculus , genetics , anatomy , in vitro
Epithelial–mesenchymal interactions are very important in the development of the vertebrate gut. In the avian embryonic stomach (proventriculus), expression of embryonic chick pepsinogen (ECPg) gene, which is specific to developing glandular cells in stomach epithelium, is regulated by mesenchymal influence. Molecular mechanisms of tissue‐specific transcriptional regulation of the ECPg gene and the molecular nature of the mesenchymal signals were analyzed using a combination of the classic organ culture system and gene transfer strategies. In the present review, three methods for the introduction of DNA into tissues are described: lipofection, electroporation and retroviral infection, and characteristics of each system are discussed.