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Pax‐6 expression during retinal regeneration in the adult newt
Author(s) -
Kaneko Yuko,
Matsumoto Gen,
Hanyu Yoshiro
Publication year - 1999
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1046/j.1440-169x.1999.00476.x
Subject(s) - retina , retinal regeneration , biology , inner nuclear layer , retinal , regeneration (biology) , microbiology and biotechnology , ganglion cell layer , inner plexiform layer , anatomy , in situ hybridization , ganglion , intrinsically photosensitive retinal ganglion cells , retinal ganglion cell , neuroscience , gene expression , botany , genetics , gene
The present study examined the expression of Pax‐6 during retinal regeneration in adult newts using in situ hybridization. In a normal retina, Pax‐6 is expressed in the ciliary marginal zone, the inner part of the inner nuclear layer, and the ganglion cell layer. After surgical removal of the neural retina, retinal pigment epithelial cells proliferate into retinal precursor cells and regenerate a fully functional retina. At the beginning of retinal regeneration, Pax‐6 was expressed in all retinal precursor cells. As regeneration proceeded, differentiating cells appeared at the scleral and vitreal margins of the regenerating retina, which had no distinct plexiform layers. In this stage, the expression of Pax‐6 was localized in a strip of cells along the vitreal margin of the regenerating retina. In the late stage of regeneration, when the layer structure was completed, the expression pattern of Pax‐6 became similar to that of a normal retina. It was found that Pax‐6 is expressed in the retinal precursor cells in the early regenerating retina and that the expression pattern of Pax‐6 changed as cell differentiation proceeded during retinal regeneration.