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Analysis of the origin and development of hatching gland cells by transplantation of the embryonic shield in the fish, Oryzias latipes
Author(s) -
Inohaya Keiji,
Yasumasu Shigeki,
Yasumasu Ikuo,
Iuchi Ichiro,
Yamagami Kenjiro
Publication year - 1999
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1046/j.1440-169x.1999.00456.x
Subject(s) - oryzias , biology , hatching , embryonic stem cell , embryo , embryogenesis , gastrulation , in situ hybridization , microbiology and biotechnology , andrology , anatomy , gene expression , gene , genetics , ecology , medicine
Hatching gland cells of the medaka, Oryzias latipes , have been observed to differentiate from the anterior end of the hypoblast, which seems to first involute at the onset of gastrulation. These results suggest that the hatching gland cells of medaka originate from the embryonic shield, the putative organizer of this fish. The present study investigated whether hatching gland cells really originate from the embryonic shield in the medaka. Transplantation experiments with embryonic shield and in situ hybridization detection of hatching enzyme gene expression as a sign of terminal differentiation of the gland cells were carried out. The analysis was performed according to the following processes. First, identification and functional characterization of the embryonic shield region were made by determining the expression of medaka goosecoid gene and its organizer activity. Second, it was confirmed that the embryonic shield had an organizer activity, inducing a secondary embryo, and that the developmental patterns of hatching gland cells in primary and secondary embryos were identical. Finally, the hatching gland cells as identified by hatching enzyme gene expression were found to coincide with the dye‐labeled progeny cells of the transplanted embryonic shield. In conclusion, it was determined that hatching gland cells were derived from the embryonic shield that functioned as the organizer in medaka.