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Noggin upregulates Fos expression by a calcium‐mediated pathway in amphibian embryos
Author(s) -
Leclerc Catherine,
Duprat AnneMarie,
Moreau Marc
Publication year - 1999
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1046/j.1440-169x.1999.00421.x
Subject(s) - noggin , calcium , microbiology and biotechnology , bone morphogenetic protein , calcium in biology , chemistry , voltage dependent calcium channel , downregulation and upregulation , medicine , endocrinology , biology , biochemistry , gene , organic chemistry
In amphibia, noggin, one of the neural inducers expressed in the Spemann organizer, acts by neutralizing the effects of bone morphogenetic protein‐4 (BMP‐4). It is shown that noggin is able to activate L‐type calcium channels. The fos proto‐oncogene is known to be induced within minutes by calcium signaling. Here it is reported that in animal cap explants of the amphibian Pleurodeles waltl , noggin can induce upregulation of a FOS‐related protein in a calcium‐dependent manner. Specific inhibition of the dihydropyridine sensitive L‐type calcium channels blocked both calcium influx and the induction of FOS‐related protein. When animal cap explants were treated with caffeine in order to release calcium from an internal store or with a specific agonist of the L‐type calcium channels, FOS‐related protein could be detected in cell nuclei by 5 or 15 min, respectively. Additionally, the calcium calmodulin kinase inhibitor, KN62, could block the upregulation of FOS‐related protein induced by agents that increased intracellular calcium ([Ca 2+ ] i ). The present results suggest that transcription factors from the FOS family are downstream targets of neural inducer noggin.