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Detection of phospholipase Cγ in sea urchin eggs
Author(s) -
Nadai Céline,
Cailliau Katia,
Epel David,
Ciapa Brigitte
Publication year - 1998
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1046/j.1440-169x.1998.t01-5-00011.x
Subject(s) - phospholipase c , sea urchin , tyrosine phosphorylation , phosphorylation , phospholipase , microbiology and biotechnology , calcium , western blot , gq alpha subunit , biology , tyrosine , biochemistry , signal transduction , chemistry , enzyme , g protein , gene , organic chemistry
Phosphorylation on tyrosine and turnover of polyphosphoinositide metabolism are rapidly stimulated after fertilization. However, the interconnection between these pathways remains to be determined. In the present paper it is demonstrated that eggs of two different sea urchin species contain tyrosine phosphorylated proteins with calcium‐sensitive phospholipase C activity. We have investigated whether phospholipase Cγ (PLCγ), characteristic of tyrosine kinase receptors, could be responsible for this activity. Western blot and immunocytochemistry performed with antibodies directed against PLCγ revealed the presence of this protein in cortical regions. It was also observed that PLCγ displayed calcium‐sensitive activity. The present results suggest that PLCγ may be part of the cascade of events leading to the calcium signal responsible for egg activation at fertilization.