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Morphogenic activity of fibroblast growth factor‐2 on primary neural precursor cells in three‐dimensional culture
Author(s) -
Hiratochi Masahiro,
Fujiwara Akiyori,
Kitani Hiroshi,
Iguchi Taisen,
Sakakura Teruyo,
Tomooka Yasuhiro
Publication year - 1998
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1046/j.1440-169x.1998.t01-5-00007.x
Subject(s) - primary (astronomy) , fibroblast growth factor , microbiology and biotechnology , primary culture , precursor cell , growth factor , biology , cell culture , in vitro , biochemistry , genetics , physics , receptor , astronomy
Mouse neural precursor cells (NPC) were dissociated from fetal heads at the 10th day of gestation. When clumps of NPC were cultured in collagen gel, they grew and reorganized neural tube‐like structures in medium containing fetal calf serum at 10% and supplemented with insulin, transferrin, cholera toxin and selenite. However, dissociated NPC died when they were cultured in collagen gel at low density in the same medium. Addition of fibroblast growth factor‐2 (FGF‐2) to this culture stimulated growth of NPC and formation of neural tube‐like structures. The requirement for FGF‐2 disappeared in high seeding density culture: they grew and formed neural tube‐like structures without FGF‐2. The structures formed in collagen gel were immunohistochemically positive against anti‐FGF‐2 antibody. The results show that the three‐dimensional culture system provides a useful tool to study the roles of FGF‐2 in morphogenesis of the central nervous system.