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Establishment of in vitro spermatogenesis from spermatocytes in the medaka, Oryzias latipes
Author(s) -
Saiki Atsusi,
Tamura Masaru,
Matsumoto Masami,
Katowgi Jun,
Watanabe Akihiko,
Onitake Kazuo
Publication year - 1997
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1046/j.1440-169x.1997.t01-2-00009.x
Subject(s) - oryzias , spermatogenesis , biology , meiosis , somatic cell , prophase , andrology , sperm , microbiology and biotechnology , genetics , gene , endocrinology , medicine
Spermatocytes of the teleost, Oryzias latipes , at meiotic prophase were cultured without contact with somatic cells. They began to divide, progressing through the meiotic divisions and differentiating into round spermatids within 48 h. The chromosome number in both the primary and secondary spermatocytes at metaphase was n = 24. In spermatids, a single flagellum was formed and the release of residual bodies was observed in vitro . The size and shape of the flagellum were the same as those seen in vivo . The expression of protamine mRNA was detected in round spermatids. This result suggests that gene expression, as well as morphological change, is regulated by the progression of spermatogenesis in cell culture. Furthermore, when the eggs of O. latipes were inseminated with germ cells cultured for 10 days, normal embryos developed and hatched out. These results suggest that the spermatocytes of O. latipes develop into fertile sperm in cell culture.