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Inhibition of gastrulation in Xenopus embryos by an antibody against a cathepsin L‐like protease
Author(s) -
Miyata Shohei,
Kubo Toshiyuki
Publication year - 1997
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1046/j.1440-169x.1997.00012.x
Subject(s) - gastrulation , embryo , xenopus , biology , microbiology and biotechnology , embryogenesis , protease , blastomere , anatomy , genetics , gene , enzyme , biochemistry
An antibody against cathepsin L‐like protease (AACLP) was injected into one cell of 2‐celled Xenopus embryos. The blastopores of AACLP‐injected embryos either did not invaginate or failed to complete invagination. As a result of this failure to complete gastrulation, the body axes could not form normally and tail bud stage embryos were bent dorsally. Embryos injected with a control antibody (CA) developed normally through the tadpole stage. Mesodermal induction was not inhibited in embryos exhibiting this AACLP‐induced gastrulation defect, but the mesodermal structure of these embryos was organized incorrectly due to the defective gastrulation during the early stages.