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Effects of 5‐azacytidine on the development of parthenogenetic mouse embryos
Author(s) -
Penkov Leonid I.,
Platonov Evgeni S.,
Mironova Oksana V.,
Konyukhov Boris V.
Publication year - 1996
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1046/j.1440-169x.1996.t01-2-00005.x
Subject(s) - blastocyst , embryo , parthenogenesis , somite , biology , andrology , in vivo , ploidy , in vitro , embryogenesis , genetics , medicine , gene
This study describes the effects of 5‐azacytidine (5‐azaC) on the development of diploid parthenogenetic embryos (PE) of CBA, C57BL/6 and (CBA × C57BL/6)F 1 mice in vitro at the 1‐cell or the blastocyst stage or in vivo after implantation. Our findings indicate that genomic imprinting is modulated by genetic background. Non‐fertilized C57BL/6 eggs form diploid parthenogenetic blastocysts at a much higher frequency than CBA eggs. Eggs from F 1 hybrid females form parthenogenetic blastocysts at an approximately intermediate level between these inbred strains of mice. C57BL/6 PE do not develop to the somite stages. In contrast, CBA PE and F 1 PE develop to various somite stages. Following administration of 5–azaC at 1.0 μmol/L in vitro at the 1‐ ‐cell stage, the number of implantations of C57BL/6 PE transferred to pseudopregnant females increased. In contrast, the number of implantations and somite F 1 PE did not significantly change following exposure to 5–azaC. However, administration of 5‐azaC at the 1‐cell stage stimulates development of somite F 1 PE. Administration of 5‐azaC at 0.2 and 1.0 μmol/L in vitro at the blastocyst stage did not change the number of implantations of C57BL/6 PE. However, the number of implantations and somite CBA PE decreased. After injection of 5azaC at 0.24mg/kg in vivo at day 8 of gestation, some F 1 PE developed to 26–35 somites compared with a maximum of 25 somites in controls. The different effects of 5‐azaC on the development of PE depend upon the mouse strain used and the stage of development.