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Detection and characterization of the cis‐element in the first intron of the Ars gene in the sea urchin
Author(s) -
Iuchi Yoshihito,
Morokuma Junji,
Akasaka Koji,
Shimada Hiraku
Publication year - 1995
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1046/j.1440-169x.1995.t01-3-00003.x
Subject(s) - hemicentrotus , sea urchin , intron , transcription (linguistics) , gene , biology , promoter , gene expression , genetics , microbiology and biotechnology , linguistics , philosophy
Cis‐active elements that are related to temporal regulation of the Ars gene expression in embryos of the sea urchin, Hemicentrotus pulcherrimus , are localized in the region between −252 b and +38b. This promoter region, however, can only determine the onset of gene transcription at a defined period in sea urchin development, and cannot determine the level of the Ars gene transcription. The element that enhances Ars promoter activity is found in the first intron of the Ars gene. The first intron element of H. pulcherrimus shows a similar level of activity when introduced in embryos of another species of sea urchin, Temnopleurus toreumaticus. The size of the first intron element is determined to be 229 b.