Premium
P2Y receptor‐mediated enhancement of permeation requires Ca 2+ signalling in vascular endothelial cells
Author(s) -
Tanaka Naoko,
Kawasaki Kumiko,
Nejime Namie,
Kubota Yoko,
Takahashi Koichi,
Hashimoto Michio,
Kunitomo Masaru,
Shinozuka Kazumasa
Publication year - 2003
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1046/j.1440-1681.2003.03893.x
Subject(s) - phospholipase c , thapsigargin , p2y receptor , agonist , receptor , endothelium , umbilical vein , chemistry , biophysics , biochemistry , intracellular , microbiology and biotechnology , biology , endocrinology , in vitro
Summary 1. We investigated the effects of 2‐methylthioATP (2meS‐ATP; a P2Y receptor agonist) on the permeation of fluorescein isothiocyanate (FITC)‐labelled dextran, transendothelial electrical resistance (TEER) and intracellular calcium levels ([Ca 2+ ] i ) in cultured endothelial cells isolated from the rat caudal artery. 2. The cellular transport of FITC‐labelled dextran was enhanced and TEER of the endothelial monolayer was reduced by 2meS‐ATP. Both these effects were prevented by pyridoxalphosphate‐6‐azophenyl‐2′,4′‐disulphonic acid, a P2Y receptor antagonist, which also inhibited the increase in [Ca 2+ ] i induced by 2meS‐ATP in endothelial cells. 3. The increase in [Ca 2+ ] i induced by 2meS‐ATP was inhibited by thapsigargin (a Ca 2+ pump inhibitor) and by U‐73122 (a phospholipase C inhibitor). 4. These findings suggested that activation of the P2Y receptor enhances the passage of material in the endothelium, which is associated with Ca 2+ signalling in endothelial cells.