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Effects Of Glycine On Glomerular Filtration Rate And Segmental Tubular Handling Of Sodium In Conscious Rats
Author(s) -
Thomsen Klaus,
Nielsen Camilla Birch,
Flyvbjerg Allan
Publication year - 2002
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1046/j.1440-1681.2002.03683.x
Subject(s) - renal function , glycine , sodium , chemistry , medicine , endocrinology , biochemistry , amino acid , organic chemistry
SUMMARY 1. Infusion of the amino acid glycine leads to an increase in effective renal plasma flow (ERPF) and glomerular filtration rate (GFR) by a mechanism that possibly involves stimulation of nitric oxide (NO). Because NO also increases proximal tubular fluid output (V prox ) by inhibition of proximal tubular Na + reabsorption and modulation of the tubuloglomerular feedback system, we hypothesized that glycine would increase V prox as measured by lithium clearance (C Li ). 2. In the first series of experiments, the effect of glycine infusion (4 mg/min) was examined in conscious, unstressed, chronically catheterized rats. In an additional series of experiments, the effect of glycine was examined under similar conditions in rats pretreated with a NO synthase (NOS) inhibitor ( N G ‐nitro‐ L ‐arginine methyl ester ( L ‐NAME), 2.5 μ g/min). 3. Glycine significantly increased ERPF (from 3268 to 4018 μ L/min per 100 g bodyweight (BW)), GFR (from 874 to 1009 μ L/min per 100 g BW), C Li (from 275 to 461 μ L/min per 100 g BW) and Na + clearance (C Na ; from 2.9 to 14.0 μ L/min per 100 g BW). Fractional excretion of lithium (FE Li ; from 32 to 46%) and C Na /C Li (from 0.99 to 2.99%) also rose, indicating inhibition of proximal and distal nephron Na + reabsorption, respectively. In the rats pretreated with L ‐NAME, similar haemodynamic and tubular responses to glycine infusion were seen, suggesting that the effects were not mediated by NO. 4. We conclude, that glycine increases ERPF and GFR and it also inhibits proximal and distal nephron Na + reabsorption leading to an increase in C Li and C Na . There was no indication that any of these effects were mediated by NO.