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Effects Of Caffeine On The Uncoupling Protein Family In Obese Yellow Kk Mice
Author(s) -
Kogure Akinori,
Sakane Naoki,
Takakura Yasuto,
Umekawa Tsunekazu,
Yoshioka Keiji,
Nishino Hoyoku,
Yamamoto Takayuki,
Kawada Teruo,
Yoshikawa Toshikazu,
Yoshida Toshihide
Publication year - 2002
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1046/j.1440-1681.2002.03675.x
Subject(s) - uncoupling protein , endocrinology , medicine , brown adipose tissue , thermogenin , thermogenesis , caffeine , skeletal muscle , white adipose tissue , chemistry , saline , biology , adipose tissue
SUMMARY 1. The hypothesis that caffeine upregulates uncoupling protein (UCP)‐1, UCP‐2 and UCP‐3 expression, which contribute to thermogenesis, was investigated in obese mice. 2. The mRNA levels of UCP‐1, ‐2 and ‐3 in brown adipose tissue (BAT), UCP‐2 in white adipose tissue (WAT), and UCP‐2 and ‐3 in skeletal muscle were measured using real‐time quantitative reverse transcription–polymerase chain reaction analysis in obese yellow KK mice 4 h after the subcutaneous administration of either 60 mg/kg caffeine or physiological saline. Plasma free fatty acids, adrenaline, noradrenaline and dopamine levels were also measured. 3. In caffeine‐injected obese mice, UCP‐1 mRNA levels were significantly increased by 1.5‐fold in BAT, UCP‐2 mRNA levels were increased by 1.8‐ and 2.5‐fold in BAT and skeletal muscles, respectively, and UCP‐3 mRNA levels were increased 1.7‐ and 3.4‐fold in BAT and skeletal muscles, respectively, compared with control mice injected with physiological saline. There was no difference in UCP‐2 mRNA levels in WAT between the two groups. 4. Plasma free fatty acids and adrenaline levels were significantly elevated in mice treated with caffeine compared with those injected with physiological saline. 5. It was concluded that caffeine upregulates the expression of UCP‐1, UCP‐2 and UCP‐3 in BAT and UCP‐2 and UCP‐3 in skeletal muscles, which may contribute to thermogenesis in obese mice.