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Altered Expression Of Vascular Natriuretic Peptide Receptors In Experimental Hypertensive Rats
Author(s) -
Lee JongUn,
Kim Sunmi,
Jung Mehye,
Oh YoonWha,
Kim Soo Wan
Publication year - 2002
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1046/j.1440-1681.2002.03647.x
Subject(s) - receptor , endocrinology , medicine , npr1 , natriuretic peptide , npr2 , chemistry , heart failure
SUMMARY 1. The aim of the present study was to determine whether the regulation of vascular natriuretic peptide receptors (NPR) is related to the local renin–angiotensin system (RAS). 2. Male Sprague‐Dawley rats were made two‐kidney, one‐clip (2K1C) and deoxycorticosterone acetate (DOCA)‐salt hypertensive to activate and inhibit the RAS, respectively. Another model of hypertension was induced by treatment with an inhibitor of nitric oxide synthesis, namely N G ‐nitro‐ L ‐arginine methyl ester ( L ‐NAME). 3. The mRNA expression of NPR‐A, NPR‐C, angiotensin‐ converting enzyme (ACE) and angiotensin AT 1 receptors was determined in the thoracic aorta by semiquantitative reverse transcription–polymerase chain reaction. The particulate guanylyl cyclase activity stimulated by atrial natriuretic peptide (ANP) was also determined in the membrane fraction of the thoracic aorta. 4. The plasma concentrations of ANP were increased significantly in the three models of hypertension. Plasma renin activity was increased in 2K1C hypertension, decreased in DOCA‐salt hypertension and not significantly altered in L ‐NAME hypertension. 5. The mRNA expression of NPR‐A and NPR‐C was decreased, whereas that of ACE and AT 1 receptors was increased in 2K1C and L ‐NAME hypertension. The mRNA expression of NPR‐A and NPR‐C was increased, whereas that of ACE and AT 1 receptors was decreased in DOCA‐salt hypertension. 6. The particulate guanylyl cyclase activity was decreased in 2K1C and L ‐NAME hypertension and increased in DOCA‐salt hypertension. 7. The vascular expression of NPR may be reciprocally regulated by local RAS activity.