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Localization Of Tachykinin Receptors And Fos‐Like Immunoreactivity Induced By Substance P In Guinea‐Pig Brain
Author(s) -
Yip Jane,
Chahl Loris A
Publication year - 2000
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1046/j.1440-1681.2000.03366.x
Subject(s) - substance p , tachykinin receptor , guinea pig , receptor , chemistry , endocrinology , medicine , neuroscience , neuropeptide , pharmacology , biology
SUMMARY 1. In the present study, a comparison was made between the distribution of tachykinin NK 1 and NK 3 receptor immunoreactivity and the distribution of Fos‐like immunoreactivity induced by the tachykinin agonist substance P (SP) in the guinea‐pig brain. 2. In agreement with results from previous studies in rat brain, NK 1 receptor‐immunoreactive neurons were found to be widely distributed throughout the brain in the striatum and in diencephalic and mesencephalic structures, while NK 3 receptor‐immunoreactive neurons were mainly in telencephalic structures. Considerable overlap was observed between NK 1 and NK 3 receptor distributions. 3. Substance P induced Fos‐like immunoreactivity (Fos‐LI) in extensive areas of the guinea‐pig brain. The induction of Fos‐LI was markedly inhibited in many areas by pretreatment with the NK 1 receptor antagonist SR 140333. The NK 3 receptor antagonist SR 142801 reduced Fos‐LI staining in fewer areas, although a reduction was observed in the cortex, striatum and hypothalamus. 4. In general, tachykinin receptors were located at sites corresponding to areas of functional activation by SP, as shown by Fos‐LI. These results extend previous studies by adding a functional dimension to tachykinin receptor localization studies.