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Identification Of New Single‐Nucleotide Polymorphisms In The Thrombin Receptor Gene And Their Effects On Coronary Artery Diseases In Koreans
Author(s) -
Park HY,
Nabika T,
Jang Y,
Kim D,
Kim HS,
Masuda J
Publication year - 2000
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1046/j.1440-1681.2000.03321.x
Subject(s) - single nucleotide polymorphism , gene , thrombin , identification (biology) , genetics , medicine , cardiology , receptor , polymorphism (computer science) , nucleotide , biology , allele , genotype , platelet , botany
SUMMARY 1. The thrombin receptor (the protease‐activated receptor‐1; PAR‐1) is located on vascular cells as well as platelets and may play important roles in atherosclerotic disorders, such as coronary artery diseases (CAD). In the present study, we searched for genetic polymorphisms of the PAR‐1 gene and evaluated their effects on CAD by association analysis. 2. We identified six polymorphisms in the 5′‐untranslated region of the PAR‐1 gene by polymerase chain reaction–single‐strand conformation polymorphism (PCR‐SSCP); five single‐nucleotide polymorphisms (SNP) at –2355 (A to G), –2333 (T to G), –1428 (G to A), –1071 (C to T) and –561 (A to G) and a simple sequence repeat (SSR) polymorphism between –1935 and –1841. Five SNP were in strong linkage disequilibrium with each other to make three major haplotypes, the frequency of which was over 90% of all possible haplotypes. 3. For association analysis, 150 patients who had CAD (CAD+), 58 subjects who had no stenosis on the coronary angiogram and 186 reference subjects who had no clinical evidence of CAD were used from the Korean population. The genotype frequencies of the SNP were in Hardy–Weinberg equilibrium, except A‐561G in CAD+. The association of these SNP as well as of the SSR with CAD was not evident. This result suggests no major roles of the PAR‐1 gene in CAD in Koreans.