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Assessment Of Gastric Emptying In The Mouse Using The [ 13 C]‐Octanoic Acid Breath Test
Author(s) -
Symonds Erin L,
Butler Ross N,
Omari Taher I
Publication year - 2000
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1046/j.1440-1681.2000.03318.x
Subject(s) - gastric emptying , breath test , yolk , medicine , gastroenterology , chemistry , chromatography , stomach , food science , helicobacter pylori
SUMMARY 1. Gastric emptying studies in small laboratory animals are hampered by the deficiency of a technique that is non‐invasive and repeatable. The aim of the present study was to adapt the non‐invasive [ 13 C]‐octanoic acid breath test, which has been validated in humans, to assess both liquid and solid gastric emptying in the mouse. 2. Gastric emptying rates were investigated for a liquid meal (Intralipid ® ; Kabi Pharmacia AB, Stockholm, Sweden; n = 7) and two solid meals (egg yolk and mouse chow; n = 7) incorporating [ 13 C]‐octanoic acid. All meals were analysed for natural enrichment of [ 13 C]. Mathematical analysis of the 13 CO 2 excretion rate allowed the determination of gastric emptying parameters. 3. Gastric emptying of Intralipid ® was more rapid than egg yolk ( P < 0.0001). Gastric emptying of mouse chow could not be assessed due to intragastric separation of [ 13 C]‐octanoic acid and natural [ 13 C] enrichment of the pellet. 4. The [ 13 C]‐octanoic acid breath test can reproducibly assess both liquid and solid gastric emptying non‐invasively in the mouse. This method can now be used to assess gastric emptying in drug studies and disease studies for which there are established mouse models.