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Nitric Oxide Production And Hepatic Dysfunction In Patients With Postoperative Sepsis
Author(s) -
Satoi Sohei,
Kamiyama Yasuo,
Kitade Hiroaki,
Kwon AHon,
Takahashi Kanji,
Wei Tu,
Inoue Tomohisa,
Takahashi Hakuo
Publication year - 2000
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1046/j.1440-1681.2000.03228.x
Subject(s) - sepsis , nitric oxide , medicine , ketone bodies , nox , gastroenterology , liver function , anesthesia , surgery , endocrinology , metabolism , chemistry , organic chemistry , combustion
SUMMARY 1. Although hepatic function is well known to deteriorate following bacterial infection, the underlying mechanisms remain poorly understood. We have previously reported that nitric oxide (NO) radical leads to a decrease in the ketone body ratio (KBR) and in ATP content due to the inhibition of mitochondrial electron transport in primary cultured rat hepatocytes. 2. To evaluate the effects of NO radical on the liver in patients with postoperative sepsis, we analysed both the stable end‐product of nitric oxide radical (NOx) as well as the arterial KBR (AKBR), which reflects liver tissue NAD + /NADH. 3. Twenty patients who had undergone general abdominal surgery and who developed postoperative sepsis were divided into two groups: (i) surviving; and (ii) non‐surviving. Blood samples were collected before the development of postoperative sepsis and every 3 days until the patient either died or was discharged from hospital. 4. Plasma NOx levels in seven patients who subsequently died became progressively higher than those in the 13 surviving patients over the clinical course of postoperative sepsis. 5. In the non‐surviving group, the AKBR was significantly lower than in surviving patients, indicating impaired hepatic function. In contrast, plasma NOx levels in non‐surviving patients were significantly higher than in surviving patients. 6. Decreases in AKBR to levels below 0.7 in non‐surviving patients followed high NOx levels. Moreover, plasma NOx levels were closely correlated with the AKBR, indicating that NO radical is associated with mitochondrial dysfunction in the liver. 7. It is likely that the overproduction of NO radical plays an important role in causing fatal metabolic disorders in patients with postoperative sepsis.