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Oxidative Status And Anti‐Oxidant Enzyme Activity During Calcium Paradox In The Rat Isolated Heart
Author(s) -
Lazou Antigone,
Seraskeris Stelios,
Tsiona Vasiliki,
Drossos George
Publication year - 2000
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1046/j.1440-1681.2000.03220.x
Subject(s) - lipid peroxidation , chemistry , calcium , glutathione , superoxide dismutase , glutathione peroxidase , oxidative phosphorylation , glutathione reductase , oxidative stress , biochemistry , medicine , endocrinology , perfusion , enzyme , biology , organic chemistry
SUMMARY 1. The effect of calcium paradox on oxidative status and the activity of anti‐oxidant enzymes were studied in the rat isolated heart. Glutathione status, sulphydryl group contents and lipid peroxidation in the myocardium, as well as the release of oxidized and reduced glutathione from the heart, were taken as indices of oxidative events. 2. Reperfusion with calcium after calcium‐free perfusion induced a significant decrease in the myocardial content of reduced and oxidized glutathione and non‐protein sulphydryl groups. At the same time, a significant release of both forms of glutathione from the heart was observed. However, the ratio of oxidized to reduced glutathione remained unchanged and was not different from control. Increased lipid peroxidation was observed only after 30 min of reperfusion with calcium. 3. Increased anti‐oxidant activity during the reperfusion period was observed. Mitochondrial Mn‐superoxide dismutase (SOD) activity was increased throughout the reperfusion period, while cytoplasmic Cu,Zn‐SOD and glutathione peroxidase activity showed a transient increase at 5 min reperfusion. 4. The results do not support an important role of oxygen free radicals in cell damage observed during calcium paradox in the rat isolated heart. Production of oxygen free radicals may occur during the reperfusion period, but the quantity produced is insufficient to exceed the anti‐oxidant capacity of the heart.