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DOWN‐REGULATION BY BUCILLAMINE OF LAMINA PROPRIA LEUCOCYTES IN THE RAT COLITIS MODEL
Author(s) -
Oshitani Nobuhide,
Matsumoto Takayuki,
Nakamura Shiro,
Arakawa Tetsuo,
Kitano Atsuo
Publication year - 1999
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1046/j.1440-1681.1999.03171.x
Subject(s) - lamina propria , colitis , medicine , immunohistochemistry , cd8 , immunology , rat model , ulcerative colitis , antigen , pharmacology , pathology , epithelium , disease
SUMMARY 1. The efficacy of bucillamine has been reported in various inflammatory models. 2. In the present study, the effects of 2 weeks administration of bucillamine on hapten‐induced experimental rat colitis were analysed. The gross morphological damage score was evaluated and the expression of CD4, CD8, CD11a, CD11b, CD25, CD54 and class II major histocompatibility complex (MHC) antigen was investigated by immunohistochemical methods. 3. Mean bodyweight was significantly increased in colitic rats given 750 μg bucillamine compared with colitic rats given vehicle (distilled water). Gross morphological damage was significantly less in colitic rats given 250 or 750 μg bucillamine compared with rats given vehicle. 4. Immunohistological studies revealed that CD4, CD11a, CD11b, CD54 and class II MHC antigen expression of infiltrating leucocytes was significantly lower in rat colonic mucosa treated with bucillamine. 5. From these findings, it appears that bucillamine may act through the down‐regulation of the activation of lamina propria leucocytes in hapten‐induced rat colitis.