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iNOS expression by activated neutrophils from patients with sepsis
Author(s) -
Tsukahara Y.,
Morisaki T.,
Kojima M.,
Uchiyama A.,
Tanaka M.
Publication year - 2001
Publication title -
anz journal of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.426
H-Index - 70
eISSN - 1445-2197
pISSN - 1445-1433
DOI - 10.1046/j.1440-1622.2001.02025.x
Subject(s) - sepsis , lipopolysaccharide , medicine , nitric oxide synthase , systemic inflammatory response syndrome , tumor necrosis factor alpha , nitric oxide , immunology , messenger rna , stimulation , inflammation , biology , biochemistry , gene
Background : Enhanced production of nitric oxide (NO) is associated with various inflammatory diseases such as sepsis. Although activated neutrophils are presumably involved in septic organ injury, the expression of inducible nitric oxide synthase (iNOS) by these cells has not been elucidated. The authors investigated whether activated neutrophils in sepsis could induce mRNA for iNOS and produce NO. Methods : Peripheral blood neutrophils were obtained from healthy volunteers, and septic patients underwent a surgical operation. The neutrophils of the healthy volunteers were stimulated with lipopolysaccharide (LPS) and/or tumour necrosis factor‐α (TNF‐α). The iNOS expression and NO production were examined by the reverse transcription–polymerase chain reaction and Griess method, respectively. Results : Circulating neutrophils obtained from patients with sepsis expressed higher levels of iNOS mRNA than those from patients with systemic inflammatory response syndrome (SIRS). Resting neutrophils from normal controls did not express iNOS mRNA and thus did not produce NO. After in vitro stimulation with LPS and TNF‐α, the neutrophils did express iNOS mRNA and thus produce NO. Conclusion : Activated neutrophils may be one source of NO production in sepsis.