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Flow Cytometry Identification of X‐ and Y‐Chromosome‐Bearing Goat Spermatozoa
Author(s) -
Parrilla I,
Vazquez JM,
Roca J,
Martinez EA
Publication year - 2004
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1046/j.1439-0531.2003.00480.x
Subject(s) - flow cytometry , sperm , biology , y chromosome , chromosome , andrology , sorting , dna , significant difference , x chromosome , microbiology and biotechnology , genetics , gene , mathematics , algorithm , medicine , statistics
Contents Flow cytometric sorting technology was used to measure the difference in DNA content between X‐ and Y‐chromosome‐bearing spermatozoa in bucks. Spermatozoa were analysed by flow cytometry to characterize X‐ and Y‐chromosome‐bearing sperm populations and to quantify the DNA difference between them. Two symmetrical, overlapping and clearly separated peaks, corresponding to X‐ and Y‐bearing spermatozoa, were detected. The difference in fluorescence intensity between the peaks was 4.4 ± 0.03% without any significant inter‐ or intra‐animal variations. Therefore, the identification and selection of high‐purity samples of sperm populations for sex sorting is easier in bucks compared with other domestic species.