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Stored of Hsp72/Hsp73 in Germinal Vesicle‐stage Mouse Oocytes
Author(s) -
Liu CH,
Yang CC,
Lin DPC,
Wu MH,
Tsai KJ
Publication year - 2004
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1046/j.1439-0531.2003.00470.x
Subject(s) - germinal vesicle , blastocyst , heat shock protein , andrology , embryo , pronucleus , zygote , biology , oocyte , microbiology and biotechnology , cell , embryogenesis , gene , genetics , medicine
Contents Heat‐shock proteins (hsps) Hsp72 and Hsp73 are the stored maternal proteins found in mouse oocytes. Both hsps appear in mouse oocytes at germinal vesicle (GV) and metaphase II (M‐II)‐stages as previously demonstrated by immunoblotting analysis. In this report, we further determined the presences of Hsp72/Hsp73 proteins in mouse embryos at stages of 2‐pronucleus, arrested 1‐cell, 2‐cell, arrested 2‐cell, 4‐cell, arrested 4‐cell, 8‐cell to morula and blastocyst. Except for the blastocyst stage, the Hsp72/Hsp73 proteins were detectable in most embryo stages. The concentration of Hsp72/Hsp73 in GV‐stage oocytes was higher than that in M‐II‐stage oocytes, and in any stages of embryos before implantation. A dramatical increase in Hsp72/Hsp73 expression was found at the 2‐cell stage. Together with these findings, we speculated that hsps accumulated or stored earlier in the GV‐stage mouse oocytes to protect the oocytes against environmental influences acting on ovary, and hsps may be required for zygotic gene activation and provided a protective effect against apoptosis.