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Detection of Water Buffalo Sex Chromosomes in Spermatozoa by Fluorescence in situ Hybridization
Author(s) -
Révay T,
Kovács A,
Presicce GA,
Rens W,
Gustavsson I
Publication year - 2003
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1046/j.1439-0531.2003.00442.x
Subject(s) - y chromosome , water buffalo , biology , fluorescence in situ hybridization , centromere , locus (genetics) , in situ hybridization , hybridization probe , chromosome , microbiology and biotechnology , satellite dna , x chromosome , genetics , dna , gene , veterinary medicine , medicine , gene expression
Contents In order to identify X‐ and Y‐bearing spermatozoa in water buffalo by fluorescence in situ hybridization (FISH), some available probes of closely related species were examined. An X‐ and Y‐specific probe set, made from flow sorted yak chromosomes, labelled in somatic metaphases of water buffalo the whole X and Y, respectively, except their centromere regions. A cattle Y‐chromosome repeat sequence (BC1.2) showed strong signal on the telomere region of the buffalo Y‐chromosome, demonstrating the evolutionary conservation of this locus in water buffalo. In hybridization experiments with spermatozoa from five buffaloes, the yak X‐Y paint set demonstrated clear signals in more than 92% (46.8% X and 45.8% Y) of the cells. Using the cattle Y‐chromosome specific BC1.2 probe, clear hybridization signal was detected in more than 48% of the cells. Statistical analysis showed that there was no significant difference between bulls or from the expected 50 : 50 ratio of X‐ and Y‐bearing cells. The probes presented here are reliable to assess separation of X‐ and Y‐bearing spermatozoa.