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In Vitro Development of Marmoset Monkey Oocytes by Pre‐antral Follicle Culture
Author(s) -
Nayudu PL,
Wu J,
Michelmann HW
Publication year - 2003
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1046/j.1439-0531.2003.00398.x
Subject(s) - marmoset , germinal vesicle , callithrix , oocyte , biology , andrology , ovarian follicle , ovary , in vitro , antral follicle , organ culture , in vitro maturation , follicle , tissue culture , endocrinology , medicine , anatomy , microbiology and biotechnology , embryo , genetics , paleontology
Contents A technique for in vitro maturation of oocytes from small ovarian follicles of marmoset monkeys ( Callithrix jacchus ) has been developed. We employed a two‐step culture system for primary follicles (45–85 μ m) and a one‐step culture technique for secondary follicles (>85 μ m). The two‐step technique started with the culture of stromal tissue fragments for 2 days. Thereafter, mechanically isolated follicles were transferred to a culture system where they attached to the culture surface and grew for up to a further 12 days. Significant growth of the small follicles and their oocytes was only achieved with gonadotrophins in the medium. Oocytes with a mean diameter of 39 μ m from follicles <85 μ m reached a mean diameter of 90 μ m by the end of the two‐step culture. After in vitro maturation, 19% of oocytes from these follicles had progressed to the germinal vesicle breakdown (GVBD). Follicles between 85 and 170 μ m in diameter were isolated from the stroma and placed directly in the culture. Oocytes from these follicles had a mean diameter of 64 μ m. The maximum size the oocytes reached in culture was related to the age of the females (pre‐pubertal females: 102 ± 1.3 μ m; adults: 96 ± 1.4 μ m). Twenty‐seven per cent of oocytes from pre‐pubertal ovaries achieved GVBD and nearly two‐thirds of these progressed to polar body stage. From adult ovaries, only 12% progressed to GVBD and one‐third of these to polar body stage. It is possible to develop mature oocytes in vitro from marmoset secondary pre‐antral follicles (>85 μ m). From primary follicles, although near full size oocytes were developed, maturation capacity was incomplete.