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Loss of Restriction Site Dde I, used for Avian Molecular Sexing, in Oreophasis derbianus
Author(s) -
BermúdezHumarán LG,
ChávezZamarripa P,
GuzmánVelasco A,
LealGarza CH,
Montes de OcaLuna R
Publication year - 2002
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1046/j.1439-0531.2002.00362.x
Subject(s) - sexing , restriction site , biology , genetics , restriction fragment length polymorphism , gene , restriction fragment , restriction enzyme , evolutionary biology , microbiology and biotechnology , polymerase chain reaction
Contents Molecular sexing is a rapid and safe procedure for bird sex determination. Two universal methods based on the amplification of a chromo‐helicase‐DNA‐Binding 1 ( CHD ) gene region, located in both sexual chromosomes (Z and W), have been established. We found that molecular sexing of Oreophasis derbianus failed by using these two procedures. One of them is based on a restriction site located in CHD1W gene but absent in CHD1Z . The Dde I restriction site, used successfully to determine gender in several bird species, was found to be lost because of nucleotide change in O. derbianus . This change created a new restriction site, Nla III, that was successfully applied for sexing this endangered bird.