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Effect of Swim up and Percoll Treatment on Viability and Acrosome Integrity of Frozen–thawed Bull Spermatozoa
Author(s) -
Somfai T,
Bodó S,
Nagy S,
Papp ÁB,
Iváncsics J,
Baranyai B,
Gócza E,
Kovács A
Publication year - 2002
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1046/j.1439-0531.2002.00350.x
Subject(s) - percoll , acrosome , andrology , hemocytometer , semen , biology , acrosome reaction , membrane integrity , cryopreservation , sperm , centrifugation , membrane , medicine , biochemistry , embryo , microbiology and biotechnology
Contents The aim of this study was to investigate the effect of the swim up and Percoll methods to select frozen–thawed bull spermatozoa with high quality membrane and acrosomal integrity and final concentration. Semen samples from six Holstein–Friesian bulls were examined. The whole experiment was repeated three times. Before and after both treatments, spermatozoa were subjected to a double‐staining method and evaluated by brightfield light microscope using 40× dry, or 100× oil immersion objectives. The concentration of spermatozoa evaluated by haemocytometer was 8.8 × 10 7 /ml after thawing, and the percentage of live cells with intact acrosome was 45.8%. Both treatments significantly increased the proportion of live spermatozoa compared with no treatment, and the use of Percoll gradient resulted in a significantly higher percentage of living cells with an intact acrosome (88.2%) than the swim up method (69.4%). The concentration of spermatozoa after Percoll separation (9.3 × 10 6 /ml) was higher than that after the swim up method (5.8 × 10 6 /ml). These results indicate that spermatozoa with a higher viability and acrosome integrity can be obtained by Percoll separation than by the swim up method. Therefore the use of Percoll‐treated spermatozoa in IVF systems can be more expedient.