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Production of Buffalo ( Bubalus bubalis ) Embryos in vitro : Premises and Promises
Author(s) -
Nandi S,
Raghu HM,
Ravindranatha BM,
Chauhan MS
Publication year - 2002
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1046/j.1439-0531.2002.00340.x
Subject(s) - blastocyst , in vitro fertilisation , biology , bubalus , embryo , embryo transfer , zygote , andrology , ice calving , in vitro maturation , in vitro , human fertilization , oocyte , embryogenesis , anatomy , microbiology and biotechnology , genetics , pregnancy , lactation , medicine , ecology
Contents Techniques for in vitro production (IVP) of buffalo embryos adopting the procedures developed in cattle have received increasing interest in the recent times. A high oocyte maturation, fertilization and cleavage rate and a low rate of blastocyst yield and calving following transfer of in vitro produced buffalo embryos have been obtained. The efficiency of IVP in buffalo is much lower than that in cattle. Several problems need to be resolved before IVP technology can be used regularly in buffalo breeding. This review attempts to present an overview of the different techniques used in buffalo to produce transferable embryos in vitro , namely in vitro maturation and fertilization of immature oocytes and in vitro development of the resulting cleaved embryos to the blastocyst stage before transfer. The problems associated with IVP, the possible solutions and the new biotechniques linked to IVP are discussed.