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Angiotensin Converting Enzyme in Bovine Ovarian Follicular Fluid and its Relationship with Oestradiol and Progesterone
Author(s) -
Nielsen AH,
Schauser KH,
Svenstrup B,
Poulsen K
Publication year - 2002
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1046/j.1439-0531.2002.00335.x
Subject(s) - follicular fluid , medicine , endocrinology , luteal phase , follicular phase , ovary , estrous cycle , chemistry , captopril , angiotensin converting enzyme , biology , oocyte , embryo , blood pressure , microbiology and biotechnology
Content The purpose of the present study was to identify angiotensin converting enzyme (ACE) in bovine ovarian follicular fluid and to relate the ACE activity to the phase of the oestrous cycle, pregnancy, and the follicular fluid concentrations of oestradiol and progesterone. The ACE activity was similar to that found in bovine serum and was completely inhibited by the specific ACE inhibitor captopril. The 50% inhibitory concentration (IC 50 ) was 1.4 × 10 −8 mol/l (range 0.8 × 10 −8 to 5.0 × 10 −8 mol/l; n=6), which is similar to that found in bovine and human serum. The ACE activity did not differ in the pre‐ovulatory and luteal phase, pregnancy or cystic follicles. It correlated with the follicular fluid concentration of progesterone in cycling cows ( ρ =0.476; p < 0.005; n=36), but did not correlate with the diameter of the follicles, the follicular fluid concentration of oestradiol or the ratio between the oestradiol and progesterone concentrations. The demonstration of ACE in bovine ovarian follicular fluid provides further evidence for the presence of a local renin–angiotensin system in the bovine ovary.