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Reduced Phagocytotic Activity of Macrophages in the Bovine Retained Placenta
Author(s) -
Miyoshi M,
Sawamukai Y,
Iwanaga T
Publication year - 2002
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1046/j.1439-0531.2002.00332.x
Subject(s) - placenta , andrology , acid phosphatase , cd14 , ice calving , staining , biology , stromal cell , immunohistochemistry , fetus , immune system , chemistry , enzyme , pregnancy , immunology , biochemistry , medicine , genetics , lactation , cancer research
This study was carried out to investigate the distribution of immune cells in the bovine placenta during the postpartum period and to compare these cells between normal and retained placenta. Within 1 h after normal calving, biopsy samples of placentomes were collected from 10 cows. The occurrence of retention of fetal membranes was monitored for more than 8 h post‐calving, and the samples obtained were divided into two groups: normally discharged and retained placenta (n = 5 each). Immunohistochemical procedures were utilized to detect macrophages and T lymphocytes. Numerous CD14‐positive macrophages were found in the stroma of both normal placenta and retained placenta whereas only a few CD3‐positive T lymphocytes were found in both cases. However, histochemical staining for acid phosphatase, a predominant lysozomal enzyme, revealed that almost all macrophages showed strong enzyme activity in the normally discharged placentas, whereas in retained placenta the activity of acid phosphatase was conspicuously decreased in intensity. These results indicate that there are functional differences in placental macrophages between normal and retained placenta.