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Long‐term conservation of embryogenic competence by induction and disorganization of somatic embryos in carrot
Author(s) -
Nomura K.
Publication year - 2003
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1046/j.1439-0523.2003.00865.x
Subject(s) - somatic embryogenesis , biology , subculture (biology) , somatic cell , embryo , embryogenesis , botany , daucus carota , microbiology and biotechnology , genetics , gene
A simple procedure was established to maintain the rate of somatic embryogenesis in carrot cell strains. Secondary cell strains were induced by the disorganization of heart‐shaped somatic embryos by transferring them into a medium containing 2,4‐dichlorophenoxyacetic acid and the rate of somatic embryogenesis was restored to the original level. It was possible to keep the original rate of embryogenesis by repeated induction and disorganization of somatic embryos, as the restored rate of embryogenesis decreased gradually during subcultures. In the carrot cell suspension culture used in this work, it was possible to keep high rates of somatic embryogenesis for at least 3 years by using this method at every 50th subculture. The method presented here should be useful to maintain regeneration competence in certain important cell strains.